Part:BBa_K2036020:Design

RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1719
  Illegal AgeI site found at 1831
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2747
  

Design Notes

It is constructed to build a bi-stable system with RFP and GFP as reporters.

Source

RFP and GFP are all standard parts from the Registry, while pRM are adopted from bacteriaphage lambda.